Microdissection and microarrays
نویسندگان
چکیده
منابع مشابه
Use of laser capture microdissection, cDNA microarrays, and tissue microarrays in advancing our understanding of prostate cancer.
One difficulty in studying epithelial tumors has been the inability to isolate pure samples for DNA and RNA analysis. Prostate cancer, with its infiltrative nature, is particularly challenging. The Combination of several new technologies should help overcome these hurdles. Laser capture microdissection uses a laser beam to achieve transfer of pure cell populations for isolation of DNA, RNA, and...
متن کاملGlobal gene expression profile of nasopharyngeal carcinoma by laser capture microdissection and complementary DNA microarrays.
A number of genetic and epigenetic changes underlying the development of nasopharyngeal carcinomas have recently been identified. However, there is still limited information on the nature of the genes and gene products whose aberrant expression and activity promote the malignant conversion of nasopharyngeal epithelium. Here, we have performed a genome-wide transcriptome analysis by probing cDNA...
متن کاملComprehensive Network Analysis of Anther-Expressed Genes in Rice by the Combination of 33 Laser Microdissection and 143 Spatiotemporal Microarrays
Co-expression networks systematically constructed from large-scale transcriptome data reflect the interactions and functions of genes with similar expression patterns and are a powerful tool for the comprehensive understanding of biological events and mining of novel genes. In Arabidopsis (a model dicot plant), high-resolution co-expression networks have been constructed from very large microar...
متن کاملDifferential Gene Expression Analysis of Pure Cell Populations from Frozen Uterine Tissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays
Laser Capture Microdissection (LCM) is an innovative technique which permits the rapid and reliable procurement of pure cell populations from heterogeneous tissue sections. After LCM, RNA isolated from pure cell populations was amplified to generate adequate amounts of antisense RNA (aRNA) for hybridization, and the differential gene expression profiles were analyzed between two experimental gr...
متن کاملGene expression profiling of primary tumor cell populations using laser capture microdissection, RNA transcript amplification, and GeneChip microarrays.
Gene expression profiling from microdissected cell populations is a powerful approach to explore molecular processes involved in development and solid tumor biology. In this chapter, we detail robust and validated methods for tissue preparation and isolation of high-quality RNA from microdissected cell populations. A protocol is also provided for linear transcript amplification using as little ...
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ژورنال
عنوان ژورنال: Breast Cancer Research
سال: 2000
ISSN: 1465-542X
DOI: 10.1186/bcr-2000-66707